The Effect of Clearwater Crystals on the Germination Rate of Mung Beans


 

Researched by Emily R.
2001-02




PURPOSE

The purpose of this experiment was to determine whether Clearwater Crystals have an effect on the germination percentage of seeds.

I became interested in this idea when I saw the Clearwater Crystals at the CWF Fair, they looked interesting, and as I continued to read about them I wondered if the germination rate is actually higher and sped up.

The information gained from this experiment would be useful to farmers and gardeners, so that their crops will germinate better. 



HYPOTHESIS

My hypothesis was that seeds in soil with Clearwater Crystals would germinate faster than in soil alone.

I base my hypothesis on advertising claims of the Clearwater Crystals stating, "germination of seeds is sped up."



EXPERIMENT DESIGN

The constants in this study were:

  • the type of beans,
  • amount and type of soil,
  • source and the exposure seeds are given in light,
  • amount of tap water added each day,
  • amount of Clearwater Crystals used,
  • temperature,
  • depth each seed is planted, 
  • type and size of cups, and
  • how many seeds planted per cup.
The manipulated variable was whether the mung beans were grown with of without Clearwater Crystals in soil.

The responding variable was the percentage of germination of mung beans. 

To measure the responding variable I took count daily of how many seeds germinated. 



MATERIALS
QUANTITY ITEM DESCRIPTION
150 Mung Beans
60 Plastic Cups
8.8 Liters Unfertilized Potting Soil
1 Packet Clearwater Crystals
2 Liters Tap Water
1 Ruler



PROCEDURES
  • Gather materials on large open area.
  • Place 150 ml of potting soil per 148-ml cup.
  • Measure twelve centimeters from the bottom of each and place five mung beans spread around the cup.
  • Add 35 ml of water per cup every other day.
  • In another ten cups put 150 ml of potting soil mixed with 1/8 teaspoon Clearwater Crystals per cup. 
  • Add 35 ml of water to the Clearwater Crystal ten cups and let them set for five minutes allowing Crystals to grow.
  • Measure twelve centimeters from the bottom of each and place five mung beans spread around the cup.
  • Put these 20 cups in window seal or lighted source for 12 hours a day.
  • Record how many seeds per trial germinated daily.
  • Repeat steps 2-9 twice more. 

RESULTS

The original purpose of this experiment was to determine whether Clearwater Crystals have an effect on the germination percentage of seeds.

The results of the experiment were that Clearwater Crystals had a higher germination rate at 97.3% versus the control group, which germinated 91.3% total. 

See the table and graph below.



CONCLUSION

My hypothesis was that seeds in soil with Clearwater Crystals would germinate faster than in soil alone.

The results indicate that this hypothesis should be accepted. Clearwater Crystals had a higher germination rate than the control group. 

Because of the results of this experiment, I wonder if seeds grown in Clearwater Crystals alone would germinate any faster than soil and Clearwater Crystals. I also wonder if Clearwater Crystals with soil would grow a taller, and stronger plant. 

If I were to conduct this project again I would do each trial separately and have one hundred beans in each trial so it would be more reliable. 



REASEARCH REPORT

INTRODUCTION

For farmers and gardeners a higher germination rate is important. With a higher germination rate larger crops are grown. Plants are very important in human society they give us oxygen to breathe. They also feed humans and the animals that we eat. 

PLANT

Plant Growth

 Flowering plants have four main parts, the root, stem, leaves, and flower. The root, stem, and leaves are known as the vegetative parts of the plant and the flowers are reproductive. 
 The roots spread and absorb water and minerals underground for the plant to grow. Food for the plant is stored in the roots. The two types of root systems are the taproot and fibrous. Taproots have a main root or primary root that grows straight down, with smaller branches known as secondary roots. Fibrous root systems are when the primary root and secondary roots are all the same size.
 Stems support the plant and take sunlight and air in. Underground stem systems are subterranean and aboveground stems are aerial stems. Stems grow either above or below soil and hold the flowering reproductive parts.
 Leaves produce photosynthesis. In photosynthesis, chlorophyll absorbs light energy from the sun, through the plants leaves. This light energy combines with water, minerals, and carbon dioxide to produce sugars, other simple compounds, and chemical energy.
 The reproductive parts of the plant are found in the flower. Every flower contains a stamen; a male reproductive organ and a pistil; a female’s reproductive organ. Every stamen has an enlarged tip called an anther. The ovary forms the base of the pistil and contains the ovules. In flowering plants one spore in the ovule produces an egg cell. Two sperm cells are produced in every anther’s spores; pollen grains. For fertilization to begin a pollen grain must be transferred to the pistil of another flower of the same plant. Pollination is the name of this transfer. When the pollen grain of a flower pollinates the pistil on the same plant this is called self-pollination. If the pollen makes it to another plant’s pistil it is called cross-pollination. 

GERMINATION

Germination is the process of a seed developing into a plant. Germination begins as the seed absorbs water and swells which causes it to split. When the seed coat splits in the soil the embryo starts to root and grow the plant. An embryo has all the parts needed to make a plant. 

Germination Process

For seeds to grow they need warmth, moisture, and oxygen. Correct temperature is important. If it is too hot or cold the seed will not germinate. Moisture softens the seed coat and allows the seed to grow.  Oxygen is taken from the soil, moisture, and air. 
When the seed is germinating the structure first appears, the lower part is the hypocotyls; it develops the main root. The upper part the epicotyl grows upward and develops the stem and bud. As the embryo breaks out of the seed coat and grows downward the radicle pushes through the soil the cotyledons move apart. The upper stem rises above the ground. The stem then carries the cotyledons and bud above the ground. True leaves now develop from the bud. The cotyledons fall off as the embryo uses all their food. 

SEEDS

 A seed is a ripened ovule of a seed plant before germination. During fertilization the pollen enters the ovule through the micropyle. In flowering plants there is a hard, outer seed coat and a second thin seed coat called the tegmen. Near the micropyle is a stalk, which attaches to the placenta of the seed wall. When the seed is removed a tiny scar, the hilum, that shows the former attachment. 

Classification

All plants are divided into two group’s gymnosperms or angiosperms. Gymnosperms are not enclosed in an ovary and angiosperms are enclosed in an ovary and ripen as a pod or fruit. Seeds are also divided by whether they have one or two cotyledons. Monocotyledons have one cotyledon and dicotyledon have two. 

Seed Parts

An embryo looks like a miniature plant. Each one contains a stem, one or two cotyledons, and a plumule. The stem is attached to the cotyledons. The plumule, which produces leaves, is located at the top of the stem. 
Cotyledons are part of the embryo, they are the first leaves formed in the seed. These leaves digest and store food for the forming plant as the seed sprouts. A stem and root then develop and carry the cotyledons above the ground. As the embryo continues to grow the seed uses all its stored food for seedling growth. Cotyledons of the bean cling to the stem and develop chlorophyll and photosynthesis for the new plant. As the plant grows its new leaves from the stem the cotyledons fall off. 

PHOTOSYNTHESIS

Photosynthesis is a food making process, which occurs, in green plants. These plants combine light energy and carbon dioxide to make food. A pigment called chlorophyll absorbs the light used in photosynthesis. Chlorophyll is contained in cell bodies called chloroplasts. In chloroplasts light causes carbon dioxide with hydrogen atoms of water to form sugar. During photosynthesis oxygen is released. In photosynthetic bacteria, carbon dioxide reacts with compounds, to form sugar. The chemical formula for photosynthesis is 6CO2 + 6H20 = C6H12O6 + 6O2.

CHLOROPHYLL

Chlorophyll is the green coloring matter found in plants. Plant cells only produce chlorophyll when exposed to a light.  Living plant cells change light energy into chemical energy. These cells also take carbon dioxide from the air and it is changed to sugars or other simple organic compounds. 

BEANS

The Bean Seedling

 The bean is classified as an angiosperm and a dicotyledon. Seed coats of the bean have two structures, the hilum and the micropyle. The hilum is a tiny scar where the seed was attached to the seed stalk. The micropyle is a small hole where fertilized pollen tube entered the seed. 

Beans Nutrition

Beans, as vegetables are important for our diet because of their vitamins and minerals. They are especially rich in Vitamin A, thiamine, niacin, and Vitamin C. Important minerals found in beans are calcium, phosphorus, and iron. Peas and beans contain proteins important to our diets unlike other vegetables, it provides energy and 12-15% of the diet’s calories. In the basic seven foods group’s beans are a good source of protein and carbohydrates. 

SOIL

 Soil is an important natural resource which life depends. Plants take their nutrients from soil and animals eat these plants and get sufficient nutrients.

Soil Characteristics

The four important characteristics of soil are: color, texture, structure, and chemical conditions. 
The color of the soil is important because it allows pedologists, soil scientists, to estimate the amount of air, water, organic matter and certain elements that are in the soil. 
Texture depends on the mineral particles and determines how well water drains through a soil. Sandy soils have the largest particles and clays are microscopic. 
When soils form clumps of soil called peds, they determine the soils structure. Consistence is when soil particles and peds stick together and keep their shape. There are three main soil structures: platelike, prismlike, and blocklike. Platelike peds are thin, horizontal plates. Prismlike peds are subsoil structures in the shape of columns. Blocklike peds look like blocks with flat or curved sides. Smaller, rounded, blocks occur in topsoil and large, flat sided, blocks occur in subsoil. Smaller peds hold water and nutrients better than larger peds do.
Soils can be acid, alkaline, or neutral. The biological and chemical processes depend on the amount of acid and alkali in soil. Plants are harmed with high acid and alkali soils. Neutral soils support biological and chemical processes. 

Classification

Pedologists classify soils according to their characteristics.  The Soil Survey Staff of the United States Department of Agriculture use a system of ten groups. They are alfisols, aridisols, entisols, histosols, inceptisols, mollisols, oxisols, spodosols, ultisols, and vertisols. 
Alfisols are developed in humid climates under grasslands and forests. Some agricultural soils are alfisols. 
Aridisols contain small amounts of organic matter and occur in dry regions such as desert soil.
Entisols occur in many climates and show little development.
Histosols are organic soil that forms in water-saturated environments such as swamps and bogs.
Inceptisols are common subhumid and humid climates and are only slightly developed. 
Mollisols have thick and organically rich topsoil, which develop in prairie regions.
Oxisols have reddish color and occur in tropical regions. They are the most chemically weathered soils. 
Spodosols form in warmer, humid climates and contain iron, aluminum, and organic matter.
Ultisols are moist, well-developed, acidic soils, which occur, in humid climates.
Vertisols develop deep, wide cracks during dry, humid seasons.
 

 


BIBLIOGRAPHY

Armstrong, Joseph E. "Cotyledon." The World Book Encyclopedia. 1998. Page 1095.

Byrne, John M. "Seed." The World Book Encyclopedia. 1998. Pages 281-283.

Dwyer, Johanna T. "Nutrition." The World Book Encyclopedia. 1998. Pages 624-627, 630.

Gantt, Elisabeth. "Chlorophyll." The World Book Encyclopedia. 1998. Pages 517-518.

Johnston, Taylor J. "Soil." The World Book Encyclopedia. 1998. Pages 573-576.

Keating, Richard C. "Germination." The World Book Encyclopedia. 1998. Page 173.

"Plant." The World Book Encyclopedia. 1998. Pages 519, 529-534, 536, 538.

"Seed." Encarta Encyclopedia December 6, 2001 http://encarta.msn.com/find/Concise.asp?z=1&pg=2&ti=761557802#s5Splittstoesser, W. E. 

Wagner, David H. "Photosynthesis." The World Book Encyclopedia. 1998. Page 430.

Whitter, S. H. "Bean." The World Book Encyclopedia. 1998. Pages 180-182.

"Vegetable." The World Book Encyclopedia. 1998. Pages 316-318. 

"Vegetable." Encarta Encyclopedia December 6, 2001
http://encarta.msn.com/find/concise.asp?ti=761556865&sid=17#5
 


ACKNOWLEDGEMENTS

 This science project couldn’t have been possible without the help and assistance of several people. I would like to thank and acknowledge each of them for their help.

  • My mother and father for supporting me and driving me where I need to be.
  • Mrs. Helms for helping me with all my questions and when I was confused about what was asked of me.
  • Mr. Newkirk for letting me stay after school to finish my project and critiquing my project. 

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