The Effects of Salinity on Brine Shrimp Growth.
 
 

Researched by Shari S.
1998-99


PURPOSE

The purpose of this experiment was to determine how different levels of salinity affect size of mature brine shrimp.

I became interested in this idea when I bought a kit with brine shrimp.  The directions said to add a specific amount of salt.  I wondered if I added more salt what would happen.

The information gained from this experiment could help people who culture brine shrimp so their brine shrimp develop more efficiently.  People who feed brine shrimp to tropical fish would also benefit, because they would know how to raise the largest brine shrimp possible.


HYPOTHESIS

My hypothesis is that the growth of the brine shrimp will be positively affected by the salinity.  I think that the brine shrimp will grow larger in higher salinities.

I base my hypothesis on a statement made in the Encyclopedia of the Animal World which states that ìbrine shrimp growth depends on salinityî and ìlarger brine shrimp tend to grow in higher salinityî.


EXPERIMENT DESIGN

The constants in this study were: The amount of water added to the pans.
          The amount of eggs added to the pans.
          The amount of food added to the pans two times a week.
          The type of water. (tap)
          The temperature of the water.
           The amount of time that the brine shrimp are allowed to grow.
           The type and size of rearing pan.

The manipulated variable was the amount of salt added to the water.

The responding variable was the growth in length of the brine shrimp from ìtailî to ìheadî.

To measure the responding variable, the brine shrimp will be measured after two and one half weeks under a microscope using a micro ruler in tenths of millimeters.


MATERIALS

QUANTITY ITEM DESCRIPTION
4  Metal pans
7 quarts Tap water
1lb. Sea salt mix
15 grams Powdered yeast
16 grams Shrimp eggs
Measuring devices
 1 Scale

 
 


PROCEDURES

  1. Collect all of your materials.
  2. Label four sterile one gallon jugs 4.0, 2.0, 1.0, and 0.5
  3. Pour 3 quarts of tap water into the 4.0 jug.
  4. Pour 1 pound of sea salt into the 4.0 jug.  Let dissolve by shaking.
  5. Pour 1.5 quarts of 4.0 into 2.0 jug and dilute with 1.5 quarts of tap water.
  6.  Pour 1.5 quarts of 2.0 into 1.0 jug and dilute with 1.5 quarts of tap water
  7.  Pour 1 quart of 1.0 into 0.5 jug and dilute with 1 quart of tap water
  8. Weigh out 0.5 grams of brine shrimp eggs.
  9. Put the eggs into the 4.0 pan.
10. Repeat step 9 for each other pan.
11. Hatch eggs by maintaining temperatures of 75 to 80 degrees F. or 24-26 degrees C.
12. After the brine shrimp hatch float 0.4 grams of powdered yeast on top of water in each pan.
13. Repeat step 12 two times a week or when yeast is all gone.
14. Record brine shrimp growth for fifteen individuals in each pan. Select them randomly.
15. Measure from tip of head to tip of tail using microscope and micro ruler in millimeters.
16. Record data in table.


RESULTS

The original purpose of this experiment was to determine how different salinitys affect the growth of brine shrimp.
 
 

The results of the experiment were that the reccommended salinity (1.0) produced the largest brine shrimp.  The highest salinity (4.0) caused the brine shrimp to not even hatch.  I think this may have happened because the level of salt may have been too high for the brine shrimp to hatch in.


CONCLUSION

My hypothesis was that the salinity would positively affect the growth of the brine shrimp and that the brine shrimp in higher salinitys would grow larger.

The results indicate that this hypothesis should be rejected because the brine shrimp in the recommended salinity were the largest and the brine shrimp in the highest salinities did not even hatch.

Because of the results of this experiment, I wonder if the 4.0 salinity was too much salt and the brine shrimp were not able to hatch and were possibly killed.

If I were to conduct this project again I would have taken pictures of the brine shrimp and me conducting my experiment.



 

  RESEARCH REPORT


Introduction

Many kids purchase ìSea monkeysî in kits. ìSea monkeysî are actually brine shrimp and most kids wonder how they can grow their brine shrimp to a larger size. This report will help explain which conditions they can do this in.  This report explains the following topics:
 Brine shrimp
 Brine shrimp growth
 Salinity
 Brine shrimp vs. salinity

                                                           Brine Shrimp
Brine shrimp (scientifically known as Artemia) are a small salt-water crustacean that are closely related to the fresh water fairy shrimp. Brine shrimp are found in briny lakes or small evaporating ponds of salt- water.  Brine shrimp breath through gill plates on their small leaf like feet. Brine shrimp eat by filtering water algae and bacteria for nutrients.  Brine shrimp females lay eggs with hard shells that can be stored for long periods of time.  Females can also reproduce by parthenogenesis, which is development of eggs without fertilization. On average brine shrimp live for six months.
 
 

Brine Shrimp Growth

Brine shrimp grow up to 13 mm (or 0.5 inches) long.  They are made up of twenty segments with the last eight segments in their tail.  Male brine shrimp mature in two and one half weeks and females take three.
 
 

Salinity

Salinity is the measurement of the amount of solid matter (usually salt) in an amount of water. Salinity is measured in grams of salt per kilogram of solution.  This can be expressed in parts per thousands (ppt).
 


Salinity and Brine Shrimp

Brine shrimp growth depends on salinity and larger brine shrimp tend to grow in higher salinityís.  Brine shrimp perfer a salinity of 30-35ppt but can live in lower and higher salinityís.


Summary

In my report I explained what brine shrimp are, how they grow, what salinity is, and how salinity effects brine shrimp.  I hope you have enjoyed my research report and the rest of my project.


BIBLIOGRAPHY


"Brine Shrimp,î Encyclopedia of the Animal World, 1972. 265-266

ì Brine Shrimp,î Microsoft Encarta Encyclopedia 1998

ìBrine Shrimp Facts.î [Online] Available  http://www.brineshrimpdirect.com/art-2.html

ìLife history of the brine shrimpî [Online] Available http://www.allserv.rug.ac.be/~booghe/james/corses/artemia/arc.html

 Lochead, H. John ìBrine Shrimpî Encyclopedia Americana 1993, p. 558

Orlans, Barbara Animal Care from Protozoa to Small Mammals 1977 p.101-106

ìSalinityî Microsoft Encarta Encyclopedia 1998

Schumann, Kai ìBrine Shrimp FAQ1.1î[Online] Available http://www.thekrib.com/food/artemia-kai.html

 

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