The Effect of Various Antibiotics on Bacterial Growth

Researched by Katlin T.
2002-03




PURPOSE
 

The purpose of this experiment was to determine the effect of various antibiotics on the growth of bacteria.

I became interested in this idea when the doctor kept giving my brother antibiotics for ear infections. The antibiotics didn’t appear very effective. I wondered why. I am also interested in the medical field as a possible career.

The information gained from this experiment could benefit doctors when prescribing effective antibiotics for patients.
 
 



 

HYPOTHESIS
 

My hypothesis was that the antibiotic vancomycin would work the best. I based my hypothesis on statements by a hospital lab technician, Marie Clark. She said that in all her years of working at the hospital, vancomycin antibiotic worked the best. 
 



 

EXPERIMENT DESIGN
 

The constants in this study were:
o temperature of incubation
o place bacteria grown (incubator)
o way bacteria and antibiotics are applied
o type and size of agar plate used to grow bacteria
o amount of antibiotics
o the time kept in the incubator
o number of hours bacteria has to grow 
o grow bacteria on the same type of plate
o exposure to same bacteria
o kinds of pads used to apply antibiotic to plate
o amount of time the pads are soaked in the antibiotics

The manipulated variable was the type of antibiotic.

The responding variable was the amount of bacteria killed by the antibiotic.

To measure the responding variable the diameter of the death zone was determined in millimeters.
 



 

MATERIALS
 
 
QUANTITY
ITEM DESCRIPTION
10
agar plates
05
test tubes
05
swabs (Q- tips)
25
antibiotic discs
30
millimeters of saline
01
calipers
01
incubator
01
colorimeter



 

PROCEDURES

  1. Emulsify the bacteria type (collected from 18 to 24 hour non- selective agar plate) in sterile saline. Adjust the McFarland turbidity to .5 visually.
  2. Soak the sterile Q-tip within 1 to 5 minutes of adjusting the turbidity. Turn the Q-tip many times, pressing firmly on the inside wall of the tube above the fluid level.
  3. Swab the entire surface of the agar plate three times using the Q-tip, turning the plate 60 degrees between streaking to make sure to evenly coat the whole agar plate.
  4. Leave the agar plate lid off for 3 to 5 minutes (but less than 15 minutes) for extra surface liquid to be absorbed.
  5. Get out the chosen antibiotic discs and place on the surface of the agar plate. Add any extra discs with forceps.
  6. Reposition any disc(s) that are not flat on the agar plate.
  7. Turn plate upside-down (with lid on) and tap gently to be sure that all the discs stay on the agar surface.
  8. 8. Place the upside-down agar plate in a 35 degrees Celsius, non-carbon dioxide incubator within 15 minutes of the disc application.
  9. Incubate plates for 16 to 24 hours.
  10. Examine the plate held a few inches above a black non-reflecting background with reflected light. Measure the diameter of the zones of death using the unaided eye. Calipers, ruler, or a template is held to the back of the inverted plate for measuring to the nearest millimeter.
  11. Destroy all bacteria by using an autoclave on all contaminated items. 


 

RESULTS

The original purpose of this experiment was to determine the effect of various antibiotics on bacterial growth.

The results of the experiment showed that the antibiotic vancomycin worked the best at killing the bacteria E. coli, Suitamonaus, Staphoreaus 1, Staphoreaus 2, and Streptococcus healacta. 

See the table and graph
 



 

CONCLUSION

My hypothesis was that the antibiotic vancomycin would work the best.

The results indicate that this hypothesis should be accepted.

Because of the results of this experiment, I wonder what type of antibiotics would kill viruses the best?

If I were to conduct this project again I would have used many more plates for each type of anibiotic. I would also test more antibiotics then I did have.
 




Literary Research Report
Introduction

Health is very important to human survival and happiness. The world’s smallest living organisms, bacteria, sometimes can threaten human health. Bacteria are everywhere, inside and outside the human body. Some bacteria make people sick, and in some cases can be fatal.

Bacteria

Bacteria are simple organisms that consist of one cell lacking a nucleus. They are among the smallest living things on earth. Most bacteria measure from 0.2 to 0.3 microns. The diameter can only be seen through a microscope. Scientists classify bacteria as prokaryotes. Bacteria are widespread, present in soil, air, and water, and as parasites on and in other living things.

Bacteria were likely the only form of life on earth for over two billion years. Antony van Leeuwenhoek first observed them in the 17th century. Bacteriology as an applied science began to develop in the 19th century.

Bacteria are amazingly adaptable to varied environmental conditions. They are found in the bodies of all living organisms and on all parts of the earth and ocean depths, Artic ice and glaciers, in hot springs, and even in the stratosphere. The understanding of bacteria and their metabolic processes have been expanded by the discovery of species that can live only deep below the earth’s surface and by species that thrive without sunlight in the high temperature and pressure near hydrothermal vents on the ocean floor. There are more bacteria, as separate individuals, than any other type of organism. There can be as many as 2.5 billion bacteria in one gram of fertile soil.

Characteristics

Bacteria are grouped in a number of different ways. Most bacteria are of one of three typical shapes: rod- shaped (bacillus), round (coccus, e.g., streptococcus), and spiral (spirillum). Another group, vibrios, appears as incomplete spirals. The cytoplasm and plasma membrane of most bacterial cells are surrounded by a cell wall. The further classification of bacteria is based on cell wall characteristics. They can also be characterized by their patterns of growth, such as the chains formed by streptococci. Many bacteria, primarily the bacillus and spiral forms, are motile, swimming about by whip like movements of flagella; other bacteria have rigid rod like protuberances called "pili" that is serve as tethers.

Some bacteria can function only in the presence of oxygen. Others cannot grow in the presence of free oxygen but obtain oxygen from compounds. Facultative anaerobes can grow with or without free oxygen; obligate anaerobes are poisoned by oxygen

Pathogenic Bacteria

Bacteria that cause disease are called pathogens. Among bacterial plant diseases are leaf spot, fire blight and wilts. Animal diseases caused by bacteria include tuberculosis, cholera, syphilis, typhoid fever, and tetanus. Some bacteria attack the tissues directly; others produce poisonous substances called toxins.
Antibodies provide natural defense against harmful bacteria. Certain bacterial diseases, e.g., tetanus, can be prevented by injection of antitoxin or of serum containing antibodies against specific bacterial antigens; immunity to some can be induced by vaccination; and certain specific bacterial parasites are killed by antibiotics.

New strains of more virulent bacterial pathogens, which many   resistant to antibiotics, have appeared in recent years. Many believe this is due to the overuse antibiotics, which are both prescriptions of minor ailments.

Helpful Bacteria

Certain types of bacteria live in the intestines of humans and animals. These bacteria help in digestion and in destroying harmful organisms. Intestinal bacteria also produce some vitamins needed to help the human body.

Bacteria in soil and water play a vital role in recycling carbon, nitrogen, sulfur, and other chemical elements used by living things. Many bacteria help decompose dead organisms and animal wastes into chemical elements. Certain kinds of bacteria invert.

Antibiotics

Antibiotics are body compounds used to inhibit bacteria growth. In the beginning antibiotic referred only to natural compounds. They are produced by bacteria. Penicillin is the most familiar antibiotic and has been used to fight many infectious diseases including syphilis, gonorrhea, tetanus, and scarlet fever. Another antibiotic, streptomycin, has been used to fight tuberculosis.

Antibiotics are mainly useful for treating infections caused by bacteria. Antibiotics came into general use during the 1940’s. At the time, they were often called "wonder drugs" because they cured as many bacterial diseases that were once fatal.

Some antibiotics are effective against infections caused by fungi and protozoa. Few antibiotics are useful in treating cancer. Antibiotics are also used to treat diseases in animals. The antibiotics support the animal’s growth for reasons that are not entirely understood. Farmers sometimes add small amounts of antibiotics to their livestock feed. This worries some scientists.

Antibiotics are not effective against colds, influenza, or other viral diseases. The effectiveness of antibiotics is limited because both pathogenic microbes and cancer cells can become resistant to them.

How Antibiotics Work

Antibiotics fight microbes and cancer cells by interfering with normal cell functions. The interference occurs in one of three ways: prevention of cell wall formation, damage to cell membrane, or disruption of chemical processes.

Dangers of Antibiotics

A lot of antibiotics are regarded among the safest drugs when properly used. Antibiotics can sometimes cause dangerous side affects. The three main dangers are allergic reactions, destruction of helpful microbes, and damage to organs and tissues.

Allergic Reactions

In most cases allergic reactions are mild and produce only a rash of fever. Stern reactions can occur, and even cause death. All antibiotics are able to produce allergic reactions, but such reactions occur most often with penicillin’s. A physician usually asks if the patient has had any allergic reactions to any antibiotics before prescribing it.

Viruses

A virus is a microscopic organism that lives in a cell of another living thing. Even though viruses are extremely small they are major causes of diseases. Some viruses pass on a disease to human beings. Some diseases are measles, influenza and the common cold.

The Structure of a Virus

Viruses are not made up of cells. They lack some of the substances needed to live on their own. To find these substances, a virus must enter a cell of another living thing. It then can use the cell’s materials to live and reproduce.

A basic virus has two basic parts a core of a nucleic acid and an outer coat of protein. The core consists of either deoxyribonucleic acid or ribonucleic acid. The DNA or RNA enables the virus to reproduce after it has entered a cell.

How a Virus Infects an Organism

Most viruses reproduce in specific cells of certain organisms. When viruses cause colds reproduce in cells of the human respiratory infection area. Viruses cannot live outside their particular cells. They must be carried into the organism by air currents of some other means, and then transported by body fluids to the cells.

Viruses in Humans

Some different viruses in humans are AIDS, chickenpox, colds, cold sores, hepatitis, a liver disease, influenza, measles, mumps, poliomyelitis, rabies, and yellow fever. The nature of the disease caused by a particular type of virus is determined by which cells and tissues in the body the virus tends to invade.

How Viruses are used

Virologists study viruses mainly to learn how they cause disease and how to control these organisms. Scientists also use viruses for such purposes as insect control, cell research, and development of vaccine and other drugs.
 

resoults
 

BIBLIOGRAPHY

"Antibiotics Don’t Wear Me Out" Antibiotics. November 5, 2002      <http://www.doh.gov.uk/antibioticresistance/antibiotics2

 Cupp, Melanie Johns. "Antibiotics" <http://www.elibrary.com/s/k6/getdoc.cgi?id=242616488x127y53383w0&OIDS=0Q003D000&Form=RL&pubname=World_Book_Encyclopedia_ (2002) 

 Dale, Maureen M., B.C.H., B.M, P.H.D "Antibiotics" November 5, 2002 http://encarta.msn.com/encnet/refpages

 Goldberg, Jan. Medical Record Technician Mankato, Minnesota Capstone Press 2000 all pages used

 Glaspy, John S., etal. "Antibiotics."  TheWorld Book Encyclopedia 1998 pages 550-553

 Kahn, Jetty.   Women in Medical Science Careers. Mankato, Minnesota Capstone Press 2000 all pages used 

 Lund, Bill.  A Career in Health Care Minneapolis, Minnesota Capstone Press 1996 all pages used

 Marquis, Robert E. "Bacteria" World Book Encyclopedia 1998

 Oleksy, Walter. Science and Medicine New York, New York. Facts on File 1995 page 6,88,89

 Primm, Russell E. Medical Assistant Mankato, Minnesota Capstone Press 1998 all pages used

 Schlessinger, David. E library "Bacteria" November 6, 2002 http://www.elibrary.com/s/k6/getdoc.cgi?id=242616488x127y53383w0&OIDS=oQ002D000&Form=RL&pubname=World_Book_Encyclopedia_(2002)& 

 Simpson, Carolyn and Hall, Penelope. Careers in Medicine. New York, New York. Rosen Publishing Group Inc. 1994 all pages 
 

ACKNOWLEDGEMENTS
I would like to thank the following people for helping me with my science project:

  • Mr. Ken Newkirk for all his advice and correcting my written work.
  • Mrs. Carol Helms for helping me when I had a question or problems.
  • Mrs. Marie Clark for supervising my experiment and making it possible.
  • My mom for transporting me to and from the hospital.
Thank you!
 


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