The Effect of Temperature on the Percentage of Germination of Mung Beans

Researched by Michelle C.
2001-02



PURPOSE

The purpose of this experiment was to determine the effect of temperature on the germination percentage of Mung beans.

I became interested in this idea when my family decided to grow a garden. I wanted to know how warm the weather should be before planting seeds. 

The information gained from this experiment could help farmers or anyone who wants to grow a garden decide what temperature the soil should be before they plant their seeds to get the best germination.




HYPOTHESIS

My hypothesis was that Mung beans would germinate best in 21°C temperature. I thought 32°C would be too hot and would have a lower percent of germination and 10°C would be too cold and would have a lower percentage of germination. 

I base my hypothesis on the fact that plants need energy from sun or heat to cause photosynthesis to provide food. "Food is the fuel of germination", is what the Eyewitness Books Plant says. If it is too hot the water will evaporate.




EXPERIMENT DESIGN

The constants in this study were: 

  •  The quality of the seeds
  •  The type of seeds(Mung bean)
  •  The type of plastic cups
  •  The size of cup
  •  The size of seeds
  •  The type of paper towels
  •  The size of paper towels
  •  The length of germination time
  •  The amount of light the seeds are germinated at (dark)
  •  The amount of seeds in each group
  •  The amount of water in each group
The manipulated variable was the temperature that the seeds were germinated at. 

The responding variable was the percentage of the seeds that germinated. 

To measure the responding variable I counted how many sprouted out of the 100 in each group.




MATERIALS
 
QUANTITY ITEM DESCRIPTION
300 mung bean seeds
paper towels
plastic cups
3 pieces of plastic wrap same size as paper towels
1 100 ml graduated cylinder
1 heat pad
refrigerator
3 small boxes




PROCEDURES

1. Obtain materials.
2. Tear off three paper towels and cut three pieces of plastic wrap large enough to fit over the paper towels and cover them.
3. Place 100 seeds in the middle of each paper towel.
4. Place a piece of plastic wrap over each one.
5. Roll the paper towel up so that the seeds are a little above the middle.
6. Wrap a rubber band around the towel slightly under the seeds tighlty. 
7. Turn the heating pad on medium and put the medium metal pan on top.
8. Place 100 milliliters of water in each of the three cups.
9. Place a roll in each cup.
10. Place 1 cup in the fridge, 1 cup on the pan and 1 cup out at room temperature.
11. Put the boxes over the top of each  cup.
12. Check the cups each day, but wait 4 days to check them.
13. When you count carefully take the rubber band off of the paper towel.
14. Carefully unroll the paper towels so that you can see and count through the plastic.
15. Count every seed that has a sprout.
16. Record the data on a data sheet as you count. 
17. With a calculator average the percents out.
18. Repeat all steps 3 more times except this one. 




RESULTS

The original purpose of this experiment was to determine the effect of temperature on the germination percentage of Mung beans.

The results of the experiment were that 52% of the seeds in 32°C germinated. Of the seeds in 21°C there were 43% that germinated. Out of all the seeds in 10°C none of them germinated. 

See my table and graph.




CONCLUSION

My hypothesis was that Mung beans would germinate best in 21°C temperature. I thought 32°C would be too hot and would have a lower percentage of germination and 10°C would be too cold and would have a lower percentage of germination. 

The results indicate that this hypothesis should be rejected because the beans in 32°C had the best germination which was 52% germinated. 

Because of the results of this experiment, I wonder what would have happened if I would have used more temperatures. 

If I were to conduct this project again I would use different kinds of seeds. I would also do 5 trials instead of just 3.
 
 
 
RESEARCH REPORT

Food is one of the basic needs for all humans. Agriculture is the way society produces enough food to feed everyone.

Plants
Plants are any living thing other than an animal. Plants are multi cellular organisms. The cells include a nucleus, cell membrane, cell wall and cytoplasm. Plants need carbon dioxide gas, water and energy from sunlight to cause photosynthesis to make food. Plants are green because they have a substance called chlorophyll in them. The greener the plant the more chlorophyll Vascular plants have a system of tubes that transport food, water and other materials. The largest vascular plant on earth is the Sequoia trees. The Sequoia trees can grow to be over 110meters tall. Xylem tissue carries water and nutrients up only from the roots of the plant. The cell walls are thick; they help to support the plant. Phloem tissue transports water, nutrients and waste up and down the plant. Herbaceous stems are green and soft. Woody stems are rigid and hard. Non vascular plants don’t have xylem or phloem so they can’t grow very large. They barely ever grow more than 20centimeters tall and usually live in moist areas.

Different plants live for different amounts of time. Annuals live for one year or less. They go from seed to flower to creating seeds to dying and starting the cycle all over. Biennials live for two years. The roots, stems and leaves grow the first year. The winter stems and leaves die but the roots stay alive. They grow stems, leaves, flowers, and produce seeds and die in the second year. Perennials live more than two years. In areas with cool autumns and cold winters some perennials have dormancy.  In the autumn the leaves fall so, the stems and branches are bare in the winter. Plants that loose leaves are called deciduous. Some perennials are green all year. Pine trees loose a few of their leaves all year round that’s why it looks like they are green all year.

There are three different ways that plants reproduce. In ferns and mosses joining the male and female sex cells, gametes make plants from spores. The female plants have long thin stalks with the capsule and the spores inside. When the capsule bursts the spores come out. If the spores land on a moist surface they will develop into moss plants.

Seeds from cones are called gymnosperms because they are seed bearing plants that don’t produce flowers. The female cones are on the top of the tree and have spiral woody cones. Each scale has female gametes. Male cones are smaller and less woody. The male cones grow at the tips of the branches. Male pinecones can produce millions of pollen grains. If carried by the wind pollen grains may land on the female cones. The fertilization may take place. During the fall and winter female cones fall off the trees, when the scales open the seeds fall out. Some cones need fire or extreme warmth to open the scales. Animals or wind sometimes scatter the seeds. If the seed lands on a spot with the right conditions for growing the seed will start to turn into a new pine tree.

Flowering plants are called angiosperms. There are about 230,000 Known species of angiosperms. The parts of an angiosperm include sepals, petals, stamens and pistils. Sepals protect the young buds. Petals are soft and are usually bright colors. Petals surround the pistils and stamens. Some flowers have pistils and stamens some have one or the other. Not all angiosperms have bright petals. Grasses and some deciduous trees and shrubs don’t have bright petals. The pistil is in the center of the flower and is the female reproductive organ. The pistil has a stigma and ovary that contains the female gametes. Stamens surround the stigma and are the male reproductive organs. Stamens are filaments topped with small antlers containing male gametes. Pollination occurs when pollen from the antlers comes in contact with the stigma and moves down the pollen tube. When male and female gametes join in the ovule the embryo forms. As the embryo grows, outer layers of the ovule become tough layers and become the seed coat. The seed coat protects the embryo when the embryo is released. The seed contains the embryo, the stored food and the seed coat. The ovary surrounds the seed or the seed gets larger and develops into fruit. Fruit is the ripened ovary of a flower and contains one or more seeds. Cotyledons are the parts of a plant seed that give nutrients to the embryo after germination. Monocot seeds contain one cotyledon and dicot seeds contain two cotyledons. Monocot is short for monocotyledon and dicot is short for dicotyledon. The embryo needs stored food for germination. Cross-pollination occurs when pollen comes from another flower. Most flowering plants characteristics encourage cross-pollination such as bright colors, sugary nectars, and sweet odors. Hummingbirds, bees and moths are attracted to different flowers because of colors, nectars and smells. For example as a bee lands on a flower and goes to another the pollen gets stuck to its legs. The pollen sticks to each flower as it lands. Therefore cross-pollination is occurring. Wind also blows the pollen from flower to flower. Some gardeners collect and provide pollen using the dry tip of a paintbrush. By doing this we can grow flowers with different characteristics. 

Some problems with plants are listed here. Weeds are unhelpful plants because they kill or weaken useful plants by towering over them and blocking out the sun. Pollen can cause asthma and hay fever. Poison ivy and poison oak causes skin rashes.

Photosynthesis is how plants make food. Photosynthesis means "putting together with light." In green plants sunlight caught by chlorophyll enables carbon dioxide from the air to join with water and minerals from the soil and make food. This also releases oxygen into the air. Most photosynthesis happens in small bodies called chloroplasts that are in the cells of plant leaves. The chloroplasts contain chlorophyll which absorb sunlight. Energy from the sun splits the water molecules into hydrogen and oxygen. The hydrogen joins with the carbon from the carbon dioxide to make sugar. The sugar helps a plant make the fat, protein, starch, vitamins and other materials needed to survive.

Bean plants have compound leaves with three leaflets. The flowers look like sweet pea flowers. The seeds are large and smooth. When the beans are ripe the pod opens.

In Canada and the US the most important kind of is bean, the kidney bean. The varieties include: the red kidney, the mottled pinto, and white navy. Green shell beans are picked when they are full grown but before they are hardened. Dry beans are very rich in proteins and carbohydrates. Dry beans can be a sub for meat. Lima, Mung, and Soy beans are very important in Asia. The scarlet runner bean has large bright red flowers, they are grown for beauty. If the weather is cold the seeds may decay.

Mung beans are Native to India. They are heat loving annual requiring 120 days. They have been increased in popularity because they have been used as sprouts in oriental dishes and salads. It takes 90 days of warm weather for good yield in the garden. One cup of Mung sprouts is equal to 35 calories, 22% of the daily vitamin A, 20% of the daily vitamin C. Mung beans are used for sprouting and are canned. 15,000 to 20,000 pounds are used annually and out of that three quarters is imported to North America. Farmers are advised to inaculate with appropriate Rhizobium before sewing. Fertilize like you would fertilize field beans. Because there aren’t any herbicides registered weed management consists of choosing a clean field and inter-row cultivation. Seed-corn maggots and cut worms may cause significant injury. Some diseases that the Mung bean can get are: bacterial blight and sclerotinia . To reduce the risk of them getting a disease use disease free seed, adequate crop rotation and row-crop production. Store the picked seeds at 12% moisture. Potential quality problems include cracked seeds, frost-damaged seeds and dirt.

Some of the pests of beans are the Mexican bean beetles, aphids and Leafhoppers.
Some of the diseases of beans are anthrarnose, and powdery mildew.

Thermodynamics

Thermodynamics is a branch of physics that deals with the relations between heat and other forms of energy, and the conversion of one into the other. 

Heat is a form of energy that consists of the motion of molecules of a substance. This energy can pass from one object to another and, when absorbed, produces an increase in temperature.

The first law of thermodynamics states that energy is a system and cannot be created or destroyed. The second law of thermodynamics states that heat only moves from warmer to less warm objects.

The sun is the most important source of heat. Heat also comes from volcanoes, hotsprings(geysers). Heat comes from some chemical reactions, like oxidation. Heat also comes from friction like when you rub two rocks together it causes friction and heat. For example when you strike a match is causes friction and catches the phosphorus on fire.

Heat is one of the most important energy forms. We use heat for many things like warming homes, cooking food and heating water. Heat can make turbines spin which makes electricity. Factories melt and bend metal with heat.
 

BIBLIOGRAPHY

Boehm, Robert F. "Thermodynamics". The World Book Encyclopedia .1999.

Burnie, David. Eyewitness Books Plant. New York: Alfred A. Knopf 1989 pp. 1-10, 28, 40, 58

"Exploring Heat". John Colgreen. United Learning Niles, Illinois 1993

"Heat". Student Discovery Encyclopedia. 2000.

Hershey, David R. Plant Biology Science Projects. New York, Chichester, Brisbane, Toronto, Singapore: John Wiley & Sons, Inc. pp. 3-19, 21-24

Kyle, Jr.,William C., Rubenstein, Joeseph H. Veaa, Carolyn J. "Real Science". Columbus, 
Ohio: McGraw Hill, 2000 pp. A14-A21

"Mung bean Production" January 15, 2002 http://www.gov.mb.ca/agriculture/crops/alternativecrops/bkjolsoo.html 

"Plant". Student Discovery Encyclopedia. 2000. 

Thorndike, E.L., Barnhart, Clarence L. "Dictionary". Glenview, Illinois: Scott Foresman and 
Company 1993, 1988, 1983, 1979, 1974, 1971 

 Relf, Diane "Mung beans" January 15, 2002 http://www.ext.vt.edu/pubs/envirohort/ 

Wittwer, S.H.  "Bean". The World Book Encyclopedia .1999.
 


ACKNOWLEDGEMENTS

I  would like to thank the following people. Without their help my project would not have been possible.

  •  My mom for buying the seeds for my experiment.
  •  My family for giving me the money to buy my board and journal and the money to go to the science fair.
  •  Mrs.Helms for helping me glue on the title and encouraging me. 
  •  Mrs.Hostetler for teaching me enough about science to do this on my own.
  •  Ken Newkirk for helping me get the research I needed, helping me come up with a project title and loaning me some of the supplies I needed.

 
 


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