Does Refined Sugar or Table Salt Affect Radish Growth

Researched by LeeAnne F.
2001-02




PURPOSE

The purpose of this experiment was to determine the effect of salt and sugar, and non-purified tap water on the growth of radishes. 

I became interested in this idea when looking at similar science projects at our school display.

The information gained from this experiment will benefit farmers and the general public by educating them about harmful material to plants. 



HYPOTHESIS

My hypothesis is that the salt and sugar waters will cause those groups of radishes to not produce as well as the non-purified tap water test group. 

I base my hypothesis on research and on data that others have collected while doing similar experiments.



 EXPERIMENT DESIGN

* The constants in this study were:
* The type of radish seed (Champion)
* The amount of seeds per test group (40)
* The amount of seeds per container (20) 
* Number of containers  (6)
* Amount of containers per test group (2)
* Size of container (20cm x 20cm)
* The amount of water given every 5 days (125mL)
* The amount of salt added to its test group daily (4g)
* The amount of sugar added to its test group daily (2.2g) 
* The amount of dirt per container  (518g)
* The temperature at which they were grown (20°C)
* The amount of artificial light (12 hrs.)

The manipulated variable was the different solutions given to the plants. These solutions were non-purified tap water, sugar water, and salt water. 

The responding variable was the growth of the radishes. 

To measure the responding variable centimeters was used to determine their growth.



MATERIALS

 
QUANTITY ITEM DESCRIPTION
6 plastic containers (2 per test group)
120 Champion radish seeds
20 Radish seeds per container
2.2 g. Sugar added to sugar group’s 125mL
4 g. Salt added to salt group’s 125mL
125 mL. Water per container
518 g. Potting soil (per container)
1 Metric ruler
1 Glass measuring Pyrex
1 Measuring cup
1 Triple-Beam-Balance



 PROCEDURES

1. Label the containers with masking tape and a marker. 1 and 1A is the tap group (use a black marker to label). 2 and 2A is the salt group (use a red marker to label). 3 and 3A is the sugar group (use a purple marker to label). 

2. Add 518g of potting soil to each container for all groups. 

3. Place twenty seeds in four rows of five into every container (below seed planting chart). For each test group there are 40 seeds. 

4. Water the seeds with 125mL of non-purified tap water until they begin to sprout. Make sure they receive twelve hours of artificial light every day.
NOTE: The 125mL of water is per container. 

5. Water them every five days 

6. When the seeds have sprouted place 4.4g of salt into each of the containers 125mL of water for the salt group. In the sugar group, for the two group’s container 125mL of water, place 2g of sugar into it. Do this for these two groups every time they are watered. For containers 1 and 1A add nothing to their 125mL and water them every five days.

7. When you start to apply the sugar and salt, begin to Measure the growth of all radish plants every three days. 

8. End the experiment in nine days. 



RESULTS

The original purpose of this experiment was to determine the growth affect of sugar and salt water on radishes compared to regular water. 

The results of the experiment were that the controlled group produced the healthiest amount of radishes. Sugar group (container 3 and 3A) did well until the end of the experiment. Salt group (container 2 and 2A) had seed sprouting difficulties, so there was less information to be obtained. 

See the tables and graph below.

View  My Data and Graphs




CONCLUSION

My hypothesis was that the water containing sugar and water with salt in it would cause the sugar and salt groups not to produce as well as the controlled group.

The results indicate that this hypothesis should be accepted. The results prove that the controlled group produced the best and healthiest plants; while the salt and sugar groups had sickly seedlings. 

Because of the results of this experiment, I wonder if other minerals would cause similar affects to the radish’s growth. 

My findings should be useful to farmers and the public, because my results prove that sugar and salt are harmful to a plant’s health.

If I were to conduct this project again I would use larger containers and a lot more seeds to increase the accuracy of my results. 


RESEARCH REPORT

 

Introduction

Plants come in many varieties, sizes, shapes, and colors. They are unique structures of living matter. Humans use a large amount of plants for food and other resources. Without plants the environment would suffer because plants take in the carbon dioxide we breath out and in return give us oxygen. 

Plants

 A plant is an important structure. They consist of multi cellular eukary- their cells contain membrane- bound structures called organells. The cells of a plant are enclosed by rigid cell walls, primarily consisting of cellulose.

Plant Phyla 

 The many species of the plant kingdom are separated in divisions, or phyla. Bryophytes are an assemblage of three phyla with circa 16,000 species, mosses, liverworts, and hornworts. Bryophytes lack a well developed vascular system for the internal conduction of water and nutrients and are often referred to as nonvascular plants. They smaller bryophytes are usually adapted to dessert life. Another phyla is tracheophytes, vascular plants. Vascular tissue is internal conducting tissue for the moment of water and minerals by drawing them from the ground, into the steam and leaves and steams, roots, and storage and reproductive organs. 

Radishes

Radishes are strong and hardy plants. They can withstand a mild frost and adapt to the environment they are planted in. These plants are grown for their sharp tasting roots. Unlike most vegetables they prefer sandy soil. The radish’s color can vary from pink, red, white, yellow, and a mixture of red and white. A few common radish breeds are French Breakfast, Cherry Bell, Scarlet Globe, White Icicle, and Champion.

Photosynthesis

The most important and complex characteristic of a plant is having the ability of photosynthesis. Leaves covered in a green pigment absorb the rays of the sun to begin photosynthesis. This energy is transformed into chemical energy. This whole process is done to enable plants to make a food source, a kind of unrefined sugar. It is stored in a plant’s roots, leaves, and steam until it is needed. This sugar is also used for human’s purposes when it has gone through the refining process. 

Sugar

There are two types of sugar, monosaccharides and disacchrides. Both are white crystals in true form. Monosaccharides are the simplest in carbohydrates. Common monosaccharides include glucose and fructose. In the blood, glucose is the most important carbohydrate. Fructose, also known as levulose, is found in almost all vegetables and fruits. Disacchrides are made of two monosaccharides. Enzymes can break down Disacchrides sucrose into fructose and glucose. 

Two other common disacchrides are lactose and maltose. Lactose can be found in milk and is used in a few medicines. Maltose, which is formed by starch, is used for the production of items like baby food and bread. People use sugar mainly as an artificial sweetener in almost every food. In other foods with natural ingredients it means that the sugar has not been through the refining process. Refinement is a process where all vitamins and other nutrients are taken out of the sugar. That is why sugar can cause tooth decay. 

All green plants produce sugar but sugar beets and sugar cane produce the largest amount of sugar. The sugar that you find on a shelf comes from these two plants. This type of sugar is called sucrose. Other sources of sugar are cornstarch, milk, maple syrup, and honey. 

Sugar belongs to a group called carbohydrates. Carbohydrates provide energy for all plants and animals. When large amounts of sugar (carbohydrates) are consumed a person can become over weight. If overly large amounts are taken in the results can dangerous to the person’s health. 

Sugar is sold in several forms. It is commonly found in the form of small grains (granules). It is also ground into a powder (powdered sugar) for cake frosting. Brown sugar consists of molasses-flavored syrup and sugar. This type of sugar is used in baking. Sugar is used in the making of several products. Some are mixing cement, tanning leather, and in certain plastics. Cornstarch and also other starches are made up of various sugars. Any starch can be broken down to form individual sugars by mixing them with acid or protein molecules (enzymes). When starch is completely broken down, it produces corn syrup (used for artificial flavoring). 

Salt

Salt is a clear, brittle mineral. Mainly used to flavor and preserve food. This mineral has a wide range of uses. Most is consumed by the chemical industry. They use the salt to make several chemicals and chemical products. 

Salt is made up of sodium and chloride. Its chemical name is sodium chloride. The formula of salt is NaCI . The formula represents sodium (Na) and chlorine (CI). Salt is also called by its mineral name halite. I t normally forms clear crystals that are close in shape to perfect cubes. In some cases impurities in the salt can make its color appear to be white, gray, or yellow. Table salt (common salt) can often appear white, but is actually clear.
The source of all salt is salty water (brine). Salt is necessary to maintain a healthy life style. In the blood salt is present. Cells within the blood need salt to live and function. 

All salt comes from the ocean. The ocean is so salty because as rainwater hits the ground it dissolves minerals containing sodium and chlorine the soil on riverbanks erode away and the river carries the minerals out to the sea. 

Salt that occurs in huge, massive, hard layers beneath the Earth’s surface is classified as rock salt. Rock salt was formed by the evaporation of large parts of oceans millions of years ago. In every continent these deposits are found. The United States has such deposits in 32 states. The largest are the Salina Basin salts, in Michigan, Ohio, New York, West Virginia, and Pennsylvania. 
 

  
BIBLIOGRAPHY
Challoner, Jack. The Visual Dictionary of Chemistry. New York: DK Publishing, Inc., 1996. Pg. 24 ?25 
Crosby, Marshall R. and Raven, Peter H. "Plant Phyla." 2001 Encarta Deluxe. 
Crosby, Marshall R. and Raven, Peter H. "Plant." 2001 Encarta Deluxe. 
Dean, Walter E., Jr. "Salt." World Book Encyclopedia 1999 Pg. 72-75 
Dr. Wales, Bills. "Sodium Chloride". November 19,2001 <http://www.students.stir.ac.uk/biology/ionpot/nacl.htm>
Hugnes, George R. "Radishes." World Book Encyclopedia 1999 Pg. 99-100 
Lucas, Angela and Lucas, Derek. A Spoon Full of Sugar. England: Wayland Publishers Limited, 1982. Pg. 8, 11 -13. 
Moore, Paul R. "Sugar." World Book Encyclopedia 1999 Pg. 959 
Salt Institute. "Salt". November 21, 2001 <http://www.saltinstitute.org/15.html> 
Schoneweis, Susan D. "Growing Radishes and Table Beets, G90-1004-A". November 10,2001  <http://janr.unl.edu/pubs/hortiwlter/g10004.htm>
The Ohio State University. "Radishes, Turnips, and Rutabagas (Swede Turnips)". November 10, 2001 <http://ohioline.osu.edu/b672/b672_29.html> 
The Sugar Association. "What is Sugar?". November 13,2001 <http://www.sugar.org/> 


ACKNOWLEDGEMENTS

I would like to thank the following people for assisting me with my science project. 

* My mom took me to Norman’s Nursery to obtain my seeds and pots. She also picked me up from several after school science project sessions. 

* My dad did pictorial documentation for my project.

* My eldest sister, Amy, helped me with research on my topic.

* Mr. Newkirk helped me revise my project journal and research report. 
 
 
 


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