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What Is the Effect of Various Pollutants on Daphnia?
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Researched by Jeff H.
2000-01 |
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PURPOSE
The purpose of this experiment was to determine the effect of different
kinds and amounts of pollutants on the survival rate of daphnia.
I became interested in this idea when I heard about how easy it is to
contaminate water and how polluted some bodies of water may be. So, I thought
it would be fun and interesting to study it further.
The information gained from this experiment might help people understand
how polluted water may become and the effects it has on human life and
wildlife.
HYPOTHESIS
My hypothesis was that oil would reduce the survival rate of daphnia
more than the other pollutants used in my experimenting.
I base my hypothesis on an article about water pollution in The World
Book Encyclopedia that said: "Oil spills pollute water and damage beaches.
Oil coats fish, birds, marine mammals, and other aquatic life, killing
them." Below in another paragraph, it said: " Pesticides can be harmful
to humans and wildlife."
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EXPERIMENT DESIGN
The constants in this study were:
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amount of daphnia
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amount of pollutants
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kind of water
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amount of water
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Petri dishes
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jeweler’s loupe
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percentage of pollutants
The manipulated variable was the different kinds of pollutants used in
the experiment.
The responding variable was the percentage of the daphnia that survive
in the various pollutants.
To measure the responding variable I will count the daphnia that are
still alive in the Petri dishes.
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MATERIALS
| QUANTITY |
ITEM DESCRIPTION |
| 100 |
Daphnia |
| 150ml |
Weed killer |
| 150 mg |
Table salt |
| 150 ml |
Oil |
| 10 |
Petri dishes |
| 1 |
Jeweler’s Loupe |
| 1 |
Graduated cylinder |
| 2 |
Eyedropper |
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Tap Water |
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PROCEDURES
1. Gather all of the materials
2. To make dilutions: Use parts needed out of 100, with pollutant as
1 part, 5 parts, and .2 parts.
3. Make dilution of oil and water label it as named and #1
4. Make dilution of weed killer and water, label it as named
and #2
5. Make dilution of table salt and label it as named and #3
6. Label plain tap water as named and #4
7. Label the four petri dishes A, B, C, and D
8. Add seven daphnia to each petri dish
9. Add #1 dilution to dish A
10. Add #2 to dish B
11. Add #3 to dish C
12. Add #4 to dish D
13. Check daphnia every 30 minutes to see how many are still alive
14. To check the daphnia, put the petri dishes underneath a Jeweler’s
Loupe and see each daphnia’s heart is beating
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RESULTS
The original purpose of this experiment was to determine the effect
of different kinds and amounts of pollutants on the survival rate of daphnia.
The results of the experiment were that the oil killed the daphnia the
fastest, table salt killed them the second fastest, and weed killer was
the slowest.
See the table and graphs.
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CONCLUSION
My hypothesis was that oil would reduce the survival rate of daphnia
more than the other pollutants used in my experimenting.
The results indicate that this hypothesis should be accepted because
oil did in fact reduce the survival rate of most daphnia.
Because of the results of this experiment I wonder if the temperature
of the polluted water, if it were hotter or colder, would affect the survival
rate of daphnia.
If I were to conduct this project again I would do some things differently.
First of all, I would have definitely measured the temperature of the water
with a thermometer. It might not have made much of a difference,
but it would make the results more accurate. Another thing that I
would have done differently is counting the amount of daphnia that were
living when I did the experiment instead of counting the dead ones.
This would have just saved me some time when making my charts and graphs
because I wouldn’t have had to convert the data.
I could have also paid closer attention to the daphnia in the different
pollutants and pollution levels. I could have done this by checking
them in the various dilutions every 15 minutes or so instead of every half-hour.
If I were to conduct the experiment again I would also keep watch of
the daphnia in the control group until they all died. That would
also help to make the results more meaningful. I could have also
used much more daphnia per trial. This would also make better results.
Along with that I could have had more trials.
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RESEARCH REPORT
INTRODUCTION
The food chain is very important to the lives of humans because any
one species needs the others in order to survive. If one species dies out
because of something like pollution, it might break apart the food chain
and affect humans. Daphnia are important because they reproduce quickly,
which makes it hard for them to die off. They are near the bottom of the
food chain, therefore supporting fish and other organisms.
POLLUTION
Types
There are different types of pollution that occur almost anywhere.
First, there is water pollution, one of the most common kinds. There is
also air and soil pollution. Every single kind of pollution is dangerous
and harmful to humans and wildlife.
Water Pollution
There are also specific types of water pollution. All different
chemicals and toxins have different effects on wildlife and human life.
There are many toxic chemicals that can harm living things like bleach,
any kind of weed killer, oil, gasoline or antifreeze, de-icing road salt,
and acid rain.
Acid rain is a very dangerous pollutant. It has many terrible
effects on nature. It kills aquatic plants, mammals, fish, and also
is hazardous to humans. It can even lower the ability for fish like salmon
and trout to reproduce. Acid rain usually comes from big cities where
there are large industries. The smoke and smog in the air collects
in a cloud, and then the wind blows it. Then, when it rains, all
of the pollution in the cloud comes down as acid in the water and kills
wildlife.
When oil spills into water, animals are killed from being coated in
the thick, black liquid. Birds get their feathers coated in oil,
and can not fly, and it becomes hard for them to keep water out of their
feathers. Also, when oil coats aquatic animals, they become trapped and
cannot move. They will eventually die unless rescued by humans.
Fertilizers and sewage are also very deadly if they spill into water
and contaminate it. They cause something called eutrophication.
Eutrophication is an explosive growth of microorganisms and algae that
uses up all of the oxygen in a body of water. This causes fish to
die because of oxygen loss. The food chain could break because of this
problem, affecting water mammals.
Air Pollution
The gasses that form acid rain are also a type of air pollution.
It is toxic to humans, animals, and plants when it is in the air too.
When animals breathe in air in an area that is polluted, their lungs can
be harmed or they could get sick if exposed to it for a very long time.
That’s why many big cities (like Los Angeles) have so much pollution and
smoke in the air. Automobiles also pollute the air with their exhaust.
This all gathers in the air to create pollution or smog.
Solution
People can prevent water pollution by just making sure that they don’t
leave toxins lying around where they could leak into a storm drain.
This could pollute water. Also littering, dumping, and polluting
into water is a way people pollute a river, lake, stream, or ocean.
So not doing this is an easy way to keep water clean.
Water conservation is a way to save water. Using a little less
water for everything could save some for the future.
DAPHNIA
Crustaceans
Crustaceans are marine creatures that usually have a shell of some
sort. There are many different kinds of crustaceans. The whole
crustacean group is one of many in the arthropod family. Some other
groups in the arthropod family are millipedes, centipedes, insects, and
spiders or arachnids. Even crustaceans are broken down into a couple
of groups, the large ones and the small ones. Some of the small ones
are daphnia, brine shrimp, and fairy shrimp. All of these are very
small and somewhat difficult to see with the human eye. The other
group, the large crustaceans, consists of sow bugs, crayfish, and even
lobster and crabs. These are highly visible to humans, and lobster
and crab are eaten for food.
Daphnia Facts
Daphnia are microorganisms that live in water. Their heart and
all of their insides are visible because of a clear, transparent shell.
They have branched out arms and antennae that they filter food with.
After it is filtered, it is sent to the mouth where it is swallowed.
A daphnia's eye is very large and sensitive to light. Daphnia live
in almost any still water area, whether it's a pond or a lake. Their
predators are mostly bigger aquatic creatures. Even some small fish
eat daphnia, on an occasion of little food. Daphnia eat things like
bacteria and algae. The algae that grows in a fish tank or aquarium
is perfect food for a daphnia to eat. Baccius Coli is a type of bacteria
that is favored by daphnia. It is completely microscopic. Daphnia
also eat protozoa, euglena, and chilomonas.
There are a few different types of daphnia including Daphnia magna and
Daphnia pulex. Magna are usually about four millimeters long and
visible to the naked eye. Pulex are visible, but are very hard to
see and are usually observed with a microscope or magnifying glass. To
move through the water, daphnia sink down, then flick their arms and bounce
up towards the surface. They don’t exactly swim, but just "bounce".
Daphnia and most other crustaceans reproduce very quickly. Some
specific daphnia species can have 13 billion descendants in 60 days.
This makes it hard for them to become extinct, and therefore the food chain
would normally be stable.
THE FOOD CHAIN
Both daphnia and pollution have much to do with the food chain.
All species in the food chain depend on each other for their own survival.
For example, if one species at the bottom of the food chain dies out, then
the whole thing is affected. When toxins pollute water, they may
kill a small organism at the bottom of the food chain. When they
die off, the animals that eat them can’t and so they might die off too.
This could keep on going until it affects humans if no one stops the pollution
problem. People have to know that these things are possible so that
they can help to prevent it.
SUMMARY
Water pollution, and all other kinds of pollution, are very serious
and dangerous. They could harm and even kill all kinds of wildlife,
plants, and even humans. The food chain is important because every
single creature and organism depends on each other to survive. When
pollution kills a whole species, all of the animals up the food chain are
affected.
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BIBLIOGRAPHY
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Barss, Karen. Clean Water, New York. Chelsea House Publishers
1992 pp. 5-20, 83
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Chertow, Marian R. "Environmental Pollution," World Book Encyclopedia.
1998. Pp. 330-334
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"Daphnia" Microsoft Encarta Encyclopedia Deluxe
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Hasalm, Andrew. Rivers, Hong Kong. World Book 1996. Pp. 5-24,
120
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Likens, Gene E. "Water Pollution." World Book Encyclopedia. 1998.
Vol. 21.
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Orlans, Barbara. "Arthropoda: Crustacea and Arachnida" Animal
Care From Protozoa to Small Mammals. Addison-Wesley pp. 94-96
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"The Yakima River." [online] Available http://www.tri-cityherald.com/yakima/day1/story3.html
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Thompson, Gerald. The Pond Hong Kong. South China Printing
Co. 1984 p. 128
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ACKNOWLEDGMENTS
I would like to thank the following people who made my science project
possible:
First I would like to thank my dad for helping me throughout my entire
experiment. If he hadn’t helped me to organize everything while I
was testing, it would have been a very difficult task.
Next, I would like to thank Mr. Sweeney, Director of King County Environmental
Labs in Seattle for the daphnia pulex that were used in my experiment and
in so many others.
I would also like to thank Mr. Gunner for staying after school to let
me work on my display, and for letting me work on my project and journal
during Science Class.
Last, I would like to thank Mr. Newkirk and Mrs. Paskvale. I want
to thank Mr. Newkirk for making a Daphnia Schedule for my experiment and
for staying after school so long to let kids work on their projects and
displays. Mrs. Paskvale helped me in my journal and with my procedures.
Thank You
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